From the perspective of users, the relationship between web page delay and user experience quality during mobile web browsing is studied. By recording the changes of two kinds of physiological indexes of the user's skin electricity and ECG during the multi-type web browsing process, the time delay length corresponding to the significant difference of its eigenvalues and the length of the time delay are marked by variance analysis and t test, and the subjective feelings of the users are evaluated by the MOS rating scale. The experimental results show that the quality of user experience is different under different mobile web pages. There is a nonlinear negative correlation between the subjective experience of users when opening web pages and the delay length of web pages. When the time delay of the mobile web page is less than 1 s, the user experience is good. When the time delay is more than 1 s, the user experience quality decreases at any time in a certain time delay, and the change presents a larger individual difference. At that time, the negative experience of the user is more serious than that of 4 s.