The present situation of signage design in some tourist attractions as well as the necessity of signage design on the basis of cultural ecology is given. By using case analysis method and the interpretation method, space environment and humanities style in tourist attractions can be transmitted by signage system with rich artistic language. On the basis of the related cultural ecology theory and the specific signage design works in tourist attractions, the following four basic principles such as embodying function, integrating cultural factors, preserving environment and highlighting artistry are proposed. Signage system is an organic part of tourism resources, and also a carrier to enhance tourist experience. Signage design from the perspective of cultural ecology can fully satisfy the psychological expectation of the pubic, making the design full of humanity and vitality. The integration of cultural ecology into the signage design of tourist attractions expands the breadth and depth of the signage design, which is very beneficial to the research and development of the related discipline.