The work aims to study photosensitivity of different cigarette liners and provide a reference for optimization of the accuracy of machine vision detection to improve cigarette packaging quality. Through RGB and HSV color modes, optical reaction parameters of different colors and types of liners were tested based on machine vision, and significance of difference was analyzed by regression variance analysis. After confirming the degree of significance, correlation analysis was performed to obtain the relationship between different liners and photosensitivity, and verify the photosensitivity of defective liner. There were significant differences in photosensitivity between cigarette liners of different colors and types. Among them, R not only had the greatest influence on the photosensitivity in different colors, but also had a significant positive correlation with colors. Its photosensitivity was as follows: red>light gold> silver>gold. V not only had the greatest influence on the photosensitivity in different types, but also had a significant positive correlation with types. Its photosensitivity was as follows: embossed composite foil type>ordinary transfer type>direct aluminum-plated foil type>composite foil type>laser type>aluminum vacuum transfer type. There are differences in the photosensitivity of different cigarette liners. In actual production, companies can optimize the range of photosensitivity for different brands of cigarette liner, and adjust optical parameters R and V to judge and reject defective liner.