The work aims to introduce the research progress of effects of high-concentration CO2 on the postharvest ultrastructure and physiological metabolism of berry and provide new idea and basis for its preservation. Starting from the application of modified atmosphere storage of high-concentration CO2 in the preservation of berry and the effects of treatment by modified atmosphere storage of high-concentration CO2 on the physiological and biochemical change of berry, the relevant literature on the berry, berry-like fruit and other fruit stored in high-concentration CO2 at home and abroad was summarized. By summarizing the internal mechanism and generic scientific problems of the effects of high-concentration CO2 on the postharvest ultrastructure and physiological metabolism of the berry, the underlying cause for the resistance of berry-like fruit against the high-concentration CO2 was analyzed, and the physiological mechanism of high-concentration CO2 treatment used to delay the physiological metabolism and aging process of the berry was concluded. As a kind of storage method that could effectively maintain the quality of berries through the physical means, the preservation technology of high CO2 modified atmosphere storage is characterized by the abilities to inhibit after-ripening and senescence, avoid chilling damage and other physiological disorders, and effectively prolong the shelf life of berries, etc. The comprehensive application of high concentration CO2 modified atmosphere storage and package will bring new vitality and hope for the storage and transportation of berry and its logistics distribution.