The work aims to establish a new method for the determination of the migration amounts of phenol and bisphenol A (BPA) in beer cans and apply such method in the contaminant detection of commercially available canned beer. The ethyl alcohol (volume fraction: 10%) was selected as the food simulant to carry out migration studies when the migration temperature was at 60 ℃ and the migration time was 10 days. The migration sample solution was detected by high performance liquid chromatography-fluorescence. In the proposed method, phenol and BPA were separated completely in 7 min. The limit of detection (LOD) of the method was 6.0 μg/L for phenol and 1.5 μg/L for BPA. The correlation coefficient in the range of 20~400 μg/L (mass concentration) was 0.9999 for phenol and 0.9998 in the range of 5~100 μg/L (mass concentration) for BPA. The spiked recovery of phenol was 90.0%~98.5% with RSDs ranging from 2.7% to 6.2% while that was 84.0%~102% with RSDs ranging from 3.2% to 5.3% for BPA. The method was further applied to the migration detection of phenol and BPA in 7 kinds of canned beers. Phenol was not detectable, while the detected migration amounts of BPA varied in the range of 6.9~14.8 μg/kg. With high accuracy and good sensitivity, the method can meet the detection demands of phenol and BPA migration amounts in beer cans.