The work aims to prepare chitosan/gelatin active films with hydrophobic function. The effects of gelatin-chitosan with different mass concentrations ratios on the film properties were studied to determine the best mass concentrations ratio of the two. The effect of tributyl citrate, glycerol and Tween-20 concentration on the hydrophobic properties of the active films was also studied. The preparation technology of active hydrophobic films was optimized based on the design principle of Box-Benhnken test, with tensile strength of the film as the response value. The interaction of various factors was explored to determine the optimum conditions for the preparation of chitosan-gelatin active films. The best mass concentrations ratio of gelatin/chitosan was 1∶1. The significant effect of three factors on tensile strength of films successively followed by tributyl citrate > glycerol > Tween-20. After verification, during the preparation of active films, the best mass concentrations of tributyl citrate, glycerol and Tween-20 were respectively 0.9, 0.3 and 0.1g/mL. Under this condition, the tensile strength of film was 20.9 MPa, and the elongation at break was 41.34%. The sensory, physical and hydrophobic properties of the active films prepared under this process are favorable.