The work aims to fabricate a micro-lens array anti-counterfeiting film with a high number of lines (more than 200 lines) to break through the situation that the micro-lens array anti-counterfeiting film fabricated in the domestic printing and packaging industry is the dot domestic printing and packaging industry with less than 200 lines. With polycarbonate as raw material, the laser micro-engraving technology was used to fabricate the mold through the roll-to-roll and rotary fabrication process. The embossing temperature was 139 °C and the speed was 5 m/min. A positive hexagonal hemispherical micro-lens array structure anti-counterfeiting film was fabricated. The side length of the micro-lens was 32 μm, the pitch was 48 μm and the film thickness was 121 μm. According to the relationship between the micro-lens array structure parameters, the converted grating lines were 529 lines. The fabrication of micro-lens array anti-counterfeiting film with a high number of lines and high filling factor is achieved. A method for the fabrication of micro-lens array anti-counterfeit film is provided, which is simple and suitable for mass production.