To improve the thermal insulation performance of the incubator, design and optimize the incubator structure of frozen seafood products. With respect to the transportation demand of "small batch volume and multi-batches" of frozen seafood products, an incubator for the transportation of frozen seafood products was designed. At the same time, the transient thermal analysis was conducted with Ansys Workbench to get the temperature field change in the incubator; and the incubator structure was improved by analyzing the results of the temperature field simulation. Finally, the simulation reliability was verified by means of comparison on the experimental and numerical simulations. Adding a small amount of thermal insulation materials at the corners of the incubator could enhance 9.9% of the thermal insulation performance of the incubator. The incubator structure improved by means of numerical simulation is reliable.