The work aims to develop a carton that can prolong the preservation time for bananas and kiwis at room temperature without any other fresh-keeping equipment, so as to provide a convenient and cheap fresh-keeping method for fruit and vegetable. The 1-MCP gas was loaded in the nano microspheres self-assembled by cyclodextrin to form the microsphere preservative. Then, the amount and release rate of 1-MCP in microspheres were tested. The fresh-keeping tests of bananas/kiwis in the fresh-keeping carton recombined with the preservative were conducted. The Malvern particle size analysis results indicated that, the average size of microsphere particles was 23.39 nm with the standard deviation of 1.81 nm. The test results of thermogravimetric analysis showed that, the mass fraction of 1-MCP gas loaded in the microspheres was about 2%, and the mass loss peak was between 180~210 ℃. The release rate of 1-MCP in the microsphere preservative would increase as the ambient humidity increased. The fresh-keeping carton can prolong the preservation time for the bananas and kiwis by 2~3 times under daily indoor environment.