目的 建立高效液相色谱法（HPLC）测定包装材料中3种苯扎氯铵（十二烷基二甲基苄基氯化铵、十四烷基二甲基苄基氯化铵和十六烷基二甲基苄基氯化铵）的含量。方法 将样品经甲醇-水（体积比1∶1）超声，离心过滤，二极管阵列检测器测定，外标法定量。结果 在10.0～500.0 mg/L范围内，3种苯扎氯铵的线性良好，r2≥0.9996，检出限为2.5～5.0 mg/L，定量限为10.0～20.0 mg/L，平均回收率为88.7%～96.6%，相对标准偏差为2.5%～5.3%。结论 该方法前处理简便，灵敏度和准确度高。

The work aims to establish high performance liquid chromatography (HPLC) to determine the contents of three benzalkonium chlorides (dodecyl dimethyl benzyl ammonium chloride, tetradecyl dimethyl benzyl amm- onium chloride, hexadecyl dimethyl benzyl ammonium chloride) in packaging materials. Samples were extracted by methanol-water (volume ratio 1∶1) ultrasound and centrifugal filtration, determined by diode array detector, and quantified by external standard method. The results showed that three benzalkonium chlorides had good linearity in the range of 10.0~500.0 mg/L, and r2 were less than or equal to 0.9996; the detection limit was 2.5~5.0 mg/L; the limit of quantification was 10.0 ~20.0 mg/L; the average recovery rate was 88.7%~96.6%, and the relative standard deviation was 2.5%~5.3%. The pretreatment of the proposed method is simple, and it is of high sensitivity and accuracy.