基于锐孔液滴生成原理,设计制作了生物活性物质包囊实验装置。通过嵌入毛细管取代锐孔的精密加工,实现了锐孔孔径、锐孔深度及锐孔数量的快速灵活改变。通过完善压力系统设计,实现了装置的小型化,减少了样品使用量。进行了细胞微胶囊制备实验,获得的细胞微胶囊粒径和粒径分布对压力和管径参数的微量变化敏捷,验证了该装置适用于生物活性物质包囊制备实验研究,研究了微胶囊粒径、粒径分布、产率与压力、锐孔孔径、锐孔深度等因素间的变化规律。

An experimental device for encapsulating biologically active substances was designed and fabricated based on the principle of droplets generation with orifices. Orifices were fabricated through embedding capillary instead of precision machining. Thus the parameters of orifices, such as size, depth and number, can be changed smartly. Miniaturization of the device was realized through improvement of the pressure system design, which reduces the usage amount of specimen. Cell microcapsules preparation experiment was carried out. The result showed that cell microcapsules size and size distribution varies agilely with the change of pressure and orifice diameter parameters; the device is suitable for encapsulation of biologically active substances.