采用乳化交联法制备出载银壳聚糖微球,考察了壳聚糖相对分子质量、乳化剂用量、油水比和硝酸银浓度对微球形貌和载银量、包封率的影响,并通过抑菌圈实验对其抗菌性能进行了评价。 结果表明:较低相对分子质量的壳聚糖有利于微球制备,而且所得的粒径较小,其粒径介于 1 ~ 3 μm,载银量可高至 256 mg / g,银的包封率可达 66. 8% 。 抗菌试验结果表明,无银壳聚糖微球对大肠杆菌和金黄色葡萄球菌有一定的抑菌性,加载银后的微球则具有极为显著的抗菌效果。

Silver-loaded chitosan microspheres were prepared using emulsified crosslinking method. The morphology, silver content, and encapsulation efficiency of resultant microspheres were examined to investigate the effects of chitosan molecular weight, the amount of emulsifier, ratio of oil and water, and concentration of silver nitrate. The antimicrobial performance of the chitosan microspheres was evaluated by antimicrobial experiments. The results showed that chitosan with lower molecular weight can favor the formation of microspheres and obtain smaller particle size; the diameter of chitosan microspheres is 1 to 3 μm; silver content in chitosan can reach 256 mg / g; and the encapsulation efficiency obtained is 66. 8% . Antimicrobial test results showed that the pure chitosan microspheres have certain antibacterial effect against E. coli and S. aureus and the silver-loaded chitosan microspheres bear excellent antibacterial effect.