The technology of adding bis-quaternary ammonium salt surfactant to improve the dyeing effects of bamboo split was studied. The influences of surfactant dosage, dyeing time, and dyeing temperature on the color difference of bamboo split were discussed. The dyeing process was optimized and analyzed by response surface methodology. The result showed that the surfactant can increase the color difference value. It can reach 70.13 NBS when surfactant dosage is 0.9%, the dyeing temperature is 93 ℃ and the dyeing time is 60 min; the color difference value is 15.76 NBS higher comparing with the method without surfactant.