Self-made antimicrobial-bibulous paper was used as the material for room temperature preservation experiment and was compared with other techniques. The results showed that effect of antimicrobial-bibulous paper on fresh-keeping of apple is best, and the apple’s weight loss rate, hardness, nutrient component and breathing intensity are lower than the comparison group. Antimicrobial-bibulous paper can also retain the apple’s traditional flavor and appearance, and prolong its storage period.