目的 黑枸杞富含花青苷,酸碱度的变化能改变花青苷分子核心部分的电子云分布和共轭结构,从而影响了它对光线的吸收,使其随环境pH值的变化发生可逆的颜色改变。本文利用黑枸杞花青素天然pH响应指示剂研发一种可视化的新鲜度指示膜,用于冷鲜猪肉新鲜度的检测。方法 以黑枸杞花青素为指示剂,甲基纤维素为基质、聚乙二醇-6000作为增韧剂、甘油为增塑剂制备指示膜探讨黑枸杞花青素浓度及添加量、甘油添加量以及乙醇浓度对基膜的力学性能、含水率和水溶性的影响及pH响应测定。结果 实验结果表明,LRA质量分数为25%、乙醇体积分数为45%、超声40 min时LRA得率为14.67 mg/g。含质量分数25%甲基纤维素和3 mL LRA的指示膜因为形成致密氢键网络,有最好的力学性能（拉伸强度为25.8 MPa）和阻湿性（含水率为8.2%）。LRA指示膜呈现出较好的pH响应性,能直观监测冷鲜肉的新鲜度,给冷链物流的品质控制提供一种低成本且高效的解决办法,将为可食用智能冷链可视化监测技术的推广提供参考。
Abstract
Lycium ruthenicum Murray are rich in anthocyanins. Changes in acidity and alkalinity can alter the cloud distribution of electrons and conjugated structure of the core part of anthocyanin molecules, thereby affecting their light absorption and causing reversible color changes with changes in environmental pH. The work aims to utilize the natural pH-responsive indicator property of anthocyanins of Lycium ruthenicum Murray to develop a visual freshness indicator film for detecting the freshness of chilled pork. With anthocyanins of Lycium ruthenicum Murray as the indicator, methylcellulose as the matrix, polyethylene glycol-6000 as the toughening agent, and glycerol as the plasticizer, an indicator film was prepared to study the effects of the concentration and amount of anthocyanins of Lycium ruthenicum Murray, glycerol addition, and ethanol concentration on the mechanical properties, moisture content, and water solubility of the film, as well as the pH responsiveness. The experimental results showed that at the anthocyanins of Lycium ruthenicum Murray (LRA) concentration of 25%, ethanol concentration of 45%, and 40 min of ultrasonication, the LRA yield was 14.67 mg/g. The indicator film containing 25% methylcellulose and 3 mL LRA exhibited the best mechanical properties (tensile strength of 25.8 MPa) and moisture barrier performance (moisture content of 8.2%) due to the formation of a dense hydrogen bond network. The LRA indicator film exhibits good pH responsiveness, allowing intuitive monitoring of the freshness of chilled meat, providing a low-cost and efficient solution for quality control in cold chain logistics, and offering a reference for the promotion of edible smart visual monitoring technology in cold chains.
关键词
pH比色法 /
智能指示膜 /
黑枸杞花青素 /
新鲜度检测
Key words
pH difference method /
smart indicator film /
anthocyanins of Lycium ruthenicum Murray /
freshness detection
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基金
黑龙江省自然科学基金（PL2025B006）
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