目的 以甘氨酸亚铁为指示剂,制备一种以果胶/阿拉伯胶为基质的可视化监测鱼糜新鲜度的智能包装指示膜,并探究其实际应用效果。方法 单因素实验确定甘氨酸亚铁添加量,通过硫化氢（H2S）熏蒸,比色分析与H2S敏感性分析研究膜的指示性能,同时将其应用于鱼糜的新鲜度检测。结果 选择低甲氧基果胶（LMP）与高甲氧基果胶（HMP）质量比为1∶3进行复配,甘氨酸亚铁添加量为0.2 mol/L制备薄膜,通过比色实验和硫化氢敏感性实验显示,膜颜色随膜中甘氨酸亚铁浓度的增加而发生显著变化（P<0.05）,同时在等量甘氨酸亚铁浓度的硫化氢熏蒸时,硫化氢浓度越高,膜的变色越明显（P<0.05）。结论 以甘氨酸亚铁为指示剂、果胶/阿拉伯胶为基质构建的硫化物指示膜可以对鱼糜贮藏过程中环境的硫化氢浓度做出实时响应,进而指示鱼糜在贮藏过程中的新鲜度变化。
Abstract
The work aims to fabricate a pectin/gum arabic-based intelligent packaging indicator film with ferrous glycinate as an indicator for the visual monitoring of surimi freshness and to investigate its practical application efficacy. The optimal concentration of ferrous glycinate was determined via single-factor experiments. The indicator performance of the film was evaluated through hydrogen sulfide (H2S) fumigation, colorimetric analysis, and H2S sensitivity analysis. Its application in detecting surimi freshness was subsequently assessed. Films were prepared using a blend of low methoxy pectin (LMP) and high methoxy pectin (HMP) at a ratio of 1:3, with a ferrous glycinate concentration of 0.2 mol/L. Colorimetric analysis and H2S sensitivity testing revealed that the film color changed significantly with increasing ferrous glycinate concentration (P<0.05). Furthermore, under fumigation with H2S at equivalent ferrous glycinate concentrations, higher H2S concentrations induced more pronounced color changes in the film (P<0.05). In conclusion, the sulfide indicator film, constructed with ferrous glycinate as the indicator and pectin/gum arabic as the matrix, can provide a real-time response to environmental H2S concentrations during surimi storage, thereby effectively indicating changes in surimi freshness throughout the storage period.
关键词
智能包装 /
多糖基薄膜 /
甘氨酸亚铁 /
硫化氢 /
鱼糜新鲜度
Key words
intelligent packaging /
polysaccharide-based film /
ferrous glycinate /
hydrogen sulfide /
surimi freshness
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基金
黑龙江八一农垦大学三横三纵支持计划（TDJH202003）
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