壳聚糖-&#x003b5;-聚赖氨酸/米糠蛋白复合膜的制备及性能研究

王丽爽; 智晓; 孙佳; 王娜

doi:10.19554/j.cnki.1001-3563.2025.17.015

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包装工程（技术栏目） ›› 2025, Vol. 46 ›› Issue (17) : 133-143. DOI: 10.19554/j.cnki.1001-3563.2025.17.015

先进材料

壳聚糖-ε-聚赖氨酸/米糠蛋白复合膜的制备及性能研究

王丽爽¹, 智晓², 孙佳¹, 王娜^2,*

作者信息 +

Preparation and Properties of Rice Bran Protein-chitosan-ε-polylysine Nanoparticle Composite Film

WANG Lishuang¹, ZHI Xiao², SUN Jia¹, WANG Na^2,*

Author information +

文章历史 +

摘要

目的为提升生物薄膜性能及应用价值,开发一种负载米糠蛋白-壳聚糖-ε-聚赖氨酸纳米颗粒作为改良因子的米糠蛋白复合膜（RBP-NPs）。方法采用复合热聚集自组装法制备米糠蛋白-壳聚糖-ε-聚赖氨酸纳米颗粒（RBP-NPs）,通过浇铸成膜工艺构建复合膜,并利用扫描电镜（SEM）、傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）及差示扫描量热法（DSC）表征薄膜结构,系统测试其力学性能、热稳定性、抗氧化及抗菌性能,对比分析了单一米糠蛋白膜（RBP）和RBP-NPs膜的表面形貌与结构属性、力学性能、热力学特性等,并对复合薄膜进行抗氧化性能测试和抗菌性能进行了研究。结果与RBP膜相比,RBP-NPs膜微观结构更加致密,壳聚糖及ε-聚赖氨酸与薄膜基底之间存在氢键,具有更好的相容性,使其物理特性得到显著提高,其中抗拉强度提升为1.52 MPa,断裂伸长率延伸至55.67%,且复合薄膜热变性温度由（101.11±1.07）℃升高至（105.63±1.44）℃,焓值由（6.28±0.43）J/g增加为（10.91±0.10）J/g;复合薄膜不透明度由3.90降低为3.13,透明度提升,持水性得到改进,且抗氧化性及抑菌性能均得到显著提升。结论薄膜的性能得到明显改善,为ε-聚赖氨酸的实际应用及功能性生物基薄膜的开发提供理论参考。

Abstract

The work aims to improve the performance and application value of biofilms, and develop a rice bran protein composite film (RBP NPs) with rice bran protein chitosan-ε-polylysine nanoparticles as an improvement factor. Rice bran protein-chitosan-ε-polylysine nanoparticles (RBP-NPs) were prepared by the composite thermal aggregation self-assembly method. A composite film was constructed by the casting film-forming process. The film structure was characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and differential scanning calorimetry (DSC). The mechanical properties, thermal stability, antioxidant and antibacterial properties of the film were systematically tested. The surface morphology, structural properties, mechanical properties, thermodynamic characteristics, etc. of single rice bran protein films (RBP) and RBP NPs films were compared and analyzed. The antioxidant and antibacterial properties of the composite film were tested. Compared with the RBP films, the microstructure of the RBP NPs films with the addition of composite nanoparticles was denser, and there were hydrogen bonds between the membrane substrate, resulting in better compatibility and significantly improved physical properties. The tensile strength was increased to 1.52 MPa, the elongation at break was extended to 55.67%, and the thermal denaturation temperature of the composite film increased from (101.11±1.07) ℃ to (105.63±1.44) ℃, with the enthalpy value increased from (6.28±0.43) J/g to (10.91±0.10) J/g; The opacity of the composite film decreased from 3.90 to 3.13, indicating an improvement in transparency and water holding capacity. Moreover, both the antioxidant and antibacterial properties were significantly enhanced. In conclusion, the performance of the film is significantly improved, providing theoretical reference for the practical application of ε-polylysine and the development of functional biobased films.

导出引用

王丽爽, 智晓, 孙佳, 王娜. 壳聚糖-ε-聚赖氨酸/米糠蛋白复合膜的制备及性能研究[J]. 包装工程（技术栏目）. 2025, 46(17): 133-143 https://doi.org/10.19554/j.cnki.1001-3563.2025.17.015

WANG Lishuang, ZHI Xiao, SUN Jia, WANG Na. Preparation and Properties of Rice Bran Protein-chitosan-ε-polylysine Nanoparticle Composite Film[J]. Packaging Engineering. 2025, 46(17): 133-143 https://doi.org/10.19554/j.cnki.1001-3563.2025.17.015

中图分类号： TB484 TS210.9

参考文献

[1] WU X J, WU W.Effects of Protein Oxidation Induced by Rice Bran Rancidity on the Interfacial Properties of Rice Bran Albumin[J]. Food Science, 2021, 42(6).
[2] 欧文华, 曹天翔, 张冬梅, 等. 米糠蛋白乳化性能及其改性方法研究进展[J]. 食品与机械, 2024, 40(1): 204-211.
OU W H, CAO T X, ZHANG D M, et al.Research Progress on Emulsifying Properties and Modification Methods of Rice Bran Protein[J]. Food & Machinery, 2024, 40(1): 204-211.
[3] YU Y H, GAINE G K, ZHOU L Y, et al.The Classical and Potential Novel Healthy Functions of Rice Bran Protein and Its Hydrolysates[J]. Critical Reviews in Food Science and Nutrition, 2022, 62(30): 8454-8466.
[4] ZHANG Y P, ZHANG M J, DIAO Y C, et al.Foaming Characteristics and Underlying Mechanism of Rice Bran Protein-Ovalbumin Mixtures[J]. Food Science, 2022, 43(12).
[5] 刘锦渊, 刘晓丽, 夏文水. 负载丁香酚的改性纳米粒对玉米醇溶蛋白膜性能的影响[J]. 食品科学技术学报, 2023, 41(5): 123-135.
LIU J Y, LIU X L, XIA W S.Effects of Modified Nanoparticles Loaded with Eugenol on Properties of Zein Films[J]. Journal of Food Science and Technology, 2023, 41(5): 123-135.
[6] ZHANG W, CHEN J W, CHEN Y, et al.Enhanced Physicochemical Properties of Chitosan/Whey Protein Isolate Composite Film by Sodium Laurate-Modified TiO₂ Nanoparticles[J]. Carbohydrate Polymers, 2016, 138: 59-65.
[7] LI S J, LIU X L, ZHANG X Q, et al.Preparation and Characterization of Zein-Tannic Acid Nanoparticles/Chitosan Composite Films and Application in the Preservation of Sugar Oranges[J]. Food Chemistry, 2024, 437: 137673.
[8] SAMADLOUIE H R, GHARANJIK S, TABATABAIE Z B.Optimization of the Production of ε-Poly-L-Lysine by Novel Producer Lactic Acid Bacteria Isolated from Traditional Dairy Products[J]. BioMed Research International, 2020, 2020: 2145656.
[9] LIU Z T, DONG Y P, LI Y C, et al.Antifungal Effect of Ε-Polylysine on Alternaria Alternata Isolated from Pears with Black Spot and Its Possible Mechanism[J]. Food Science, 2021, 42(11).
[10] WANG D H, WANG H M, WU J P, et al.Biotechnological Production and Application of Epsilon-Poly-L-Lysine (ε-PL): Biosynthesis and Its Metabolic Regulation[J]. World Journal of Microbiology and Biotechnology, 2022, 38(7): 123.
[11] ZHOU X J, GUAN C M, MA Q Q, et al.Elaboration and Characterization of Ε-Polylysine-Sodium Alginate Nanoparticles for Sustained Antimicrobial Activity[J]. International Journal of Biological Macromolecules, 2023, 251: 126329.
[12] LIU J, LIU S, CHEN Y, et al.Physical, Mechanical and Antioxidant Properties of Chitosan Films Grafted with Different Hydroxybenzoic Acids[J]. Food Hydrocolloids, 2017, 71: 176-186.
[13] NOSHIRVANI N, GHANBARZADEH B, GARDRAT C, et al.Cinnamon and Ginger Essential Oils to Improve Antifungal, Physical and Mechanical Properties of Chitosan-Carboxymethyl Cellulose Films[J]. Food Hydrocolloids, 2017, 70: 36-45.
[14] 潘红阳, 张根义. 不同脂类及添加量对大豆蛋白基可食性膜性能的影响[J]. 食品研究与开发, 2006, 27(1): 86-89.
PAN H Y, ZHANG G Y.Soy Protein Isolate Emulsion Film Performance as Affected by Lipid and Amount[J]. Food Research and Development, 2006, 27(1): 86-89.
[15] JU S Y, ZHANG F L, DUAN J F, et al.Characterization of Bacterial Cellulose Composite Films Incorporated with Bulk Chitosan and Chitosan Nanoparticles: A Comparative Study[J]. Carbohydrate Polymers, 2020, 237: 116167.
[16] WANG H P, GONG X C, MIAO Y L, et al.Preparation and Characterization of Multilayer Films Composed of Chitosan, Sodium Alginate and Carboxymethyl Chitosan-ZnO Nanoparticles[J]. Food Chemistry, 2019, 283: 397-403.
[17] 余紫娟. 负载原花青素的纳米颗粒/壳聚糖膜的构建及其在鱼肉保鲜中的应用[D]. 无锡: 江南大学, 2022.
YU Z J.Construction of Proanthocyanidin-Loaded Nanoparticle/Chitosan Films and Their Application in Fish Preservation[D]. Wuxi: Jiangnan University, 2022.
[18] ZHANG L M, CHEN D Y, YU D W, et al.Modulating Physicochemical, Antimicrobial and Release Properties of Chitosan/Zein Bilayer Films with Curcumin/Nisin-Loaded Pectin Nanoparticles[J]. Food Hydrocolloids, 2022, 133: 107955.
[19] MIR N A, RIAR C S, SINGH S.Effect of Film Forming Solution pH on Antibacterial, Antioxidant and Structural Characteristics of Edible Films from Modified Quinoa Protein[J]. Food Hydrocolloids, 2023, 135: 108190.
[20] 雷会宁. 大豆分离蛋白复合膜制备及应用研究[D]. 天津: 天津科技大学, 2015.
LEI H N.Preparation and Application of Soy Protein Isolate Composite Films[D]. Tianjin: Tianjin University of Science & Technology, 2015.
[21] DURMAZ B U, AYTAC A.Development and Characterization of Poly(vinyl alcohol) and Casein Blend Films[J]. Polymer International, 2019, 68(6): 1140-1145.
[22] 雷俊. 可食性膜的成膜性能及其应用研究[D]. 乌鲁木齐: 新疆农业大学, 2008.
LEI J.Study on Film-Forming Properties and Application of Edible Films[D]. Urumqi: Xinjiang Agricultural University, 2008.
[23] ZHANG J J, LI A L, CHENG J J.Progress in the Application of Maillard Reaction Modified-Casein in the Delivery of Bioactive Substances[J]. Food Science, 2022, 43(23): 1-10.
[24] CHANG C, WANG T R, HU Q B, et al.Caseinate-Zein-Polysaccharide Complex Nanoparticles as Potential Oral Delivery Vehicles for Curcumin: Effect of Polysaccharide Type and Chemical Cross-Linking[J]. Food Hydrocolloids, 2017, 72: 254-262.
[25] DONG W X, SU J Q, CHEN Y L, et al.Characterization and Antioxidant Properties of Chitosan Film Incorporated with Modified Silica Nanoparticles as an Active Food Packaging[J]. Food Chemistry, 2022, 373: 131414.
[26] BOURA-THEODORIDOU O, GIANNAKAS A, KATAPODIS P, et al.Performance of ZnO/Chitosan Nanocomposite Films for Antimicrobial Packaging Applications as a Function of NaOH Treatment and Glycerol/PVOH Blending[J]. Food Packaging and Shelf Life, 2020, 23: 100456.
[27] 郭娜, 张安坤, 卓凯丽, 等. 黑米花青素-结冷胶可食用膜的性能分析[J]. 中国食品添加剂, 2023, 34(2): 19-26.
GUO N, ZHANG A K, ZHUO K L, et al.Effect of Black Rice Anthocyanin on the Properties of Gellan Gum Edible Film[J]. China Food Additives, 2023, 34(2): 19-26.
[28] SAEDI S, RHIM J W.Synthesis of Fe₃O₄@SiO₂@PAMAM dendrimer@AgNP Hybrid Nanoparticles for the Preparation of Carrageenan-Based Functional Nanocomposite Film[J]. Food Packaging and Shelf Life, 2020, 24: 100473.
[29] ZHANG S K, HE Z Y, XU F Z, et al.Enhancing the Performance of Konjac Glucomannan Films through Incorporating Zein-Pectin Nanoparticle-Stabilized Oregano Essential Oil Pickering Emulsions[J]. Food Hydrocolloids, 2022, 124: 107222.