目的 为了监测食品贮藏和运输期间的品质，制备微生物时间-温度指示器(TTI)。方法 选择嗜酸乳杆菌作为时间-温度指示器的微生物，对配制成的9种混合pH指示剂溶液进行筛选，用合适的混合pH指示剂溶液制备TTI，加入不同浓度的嗜酸乳杆菌，在不同等温条件下(5、15、25 ℃)贮藏检测其指标，以合适的指标参数为响应值探究其动力学与温度依赖性。结果 9种混合pH指示剂溶液中有5种颜色变化较明显，用其中一种制备TTI，以色差值(∆E)为响应参数得出其活化能(Ea)为84.9 kJ/mol。结论 5种颜色变化较明显的混合pH指示剂溶液可以作为TTI的指示剂，制备的微生物型TTI有潜力应用于活化能59.9 kJ/mol≤Ea≤109.9 kJ/mol的食品品质指标的货架期预测中。

The work aims to prepare microbial time-temperature indicators (TTIs) for monitoring the quality of food products during storage and transportation. Lactobacillus acidophilus was selected as the microorganism of the time-temperature indicator, and nine mixed pH indicator solutions were prepared for screening. The TTI was prepared with suitable mixed pH indicator solutions, and the indicators were evaluated by adding different concentrations of Lactobacillus acidophilus and stored under different isothermal conditions (5, 15 and 25 ℃), and the kinetics and temperature dependence were investigated with suitable indicator parameters as response values. Five of the nine mixed pH indicator solutions had significant color changes, one of which was used to prepare the TTI, and the activation energy (Ea) was 84.9 kJ/mol with the total color difference value (∆E) as the response parameter. Five mixed pH indicator solutions with significant color changes can be used as indicators for the TTI, and the prepared microbial-type TTI has potential to be applied in shelf life prediction of food with an activation energy of quality index from 59.9 kJ/mol to 109.9 kJ/mol.