目的 研发一种可视化的新鲜度指示薄膜，探究其在花蛤新鲜度监测中的可行性。方法 以对pH敏感的蓝莓花青素为指示剂，壳聚糖为基质，通过复合明胶、乳酸链球菌素(Nisin)和迷迭香精油制备5种智能指示膜，研究其pH敏感性、颜色响应性、微观结构、阻隔特性、力学性能、含水率、水溶性、抗氧化、抗菌等特性。结果 蓝莓花青素溶液在pH值为3～12内颜色响应明显。随着膜组分的增加，薄膜微观结构的粗糙度增加，而水蒸气阻隔性能逐渐降低。Nisin和迷迭香精油的添加显著增强了抗氧化和抗菌能力。壳聚糖/Nisin/迷迭香精油–蓝莓花青素(CSNR–ATH)膜的紫外线阻隔性能较优而水溶性低。CSNR–ATH膜在花蛤的冷藏过程中能灵敏反映花蛤新鲜度的变化，复合指示膜由淡绿色变为黄绿色。结论 负载蓝莓花青素的壳聚糖基智能指示膜为花蛤新鲜度的监测提供了一种新的选择。

The work aims to develop a visual freshness indicator film and explore its feasibility in the freshness monitoring of clams. With blueberry anthocyanins sensitive to pH as the indicator and chitosan as the substrate, five smart indicator films were prepared by incorporating gelatin, Nisin, and rosemary essential oil. Then, the film properties such as pH sensitivity, color responsiveness, microstructure, barrier properties, mechanical properties, water content and water solubility and antioxidant and antibacterial properties were investigated. The color of blueberry anthocyanin solution responded significantly to pH in the range of 3-12. The roughness of the microstructure of the film increased with the addition of components, while the water vapor barrier properties gradually decreased. The addition of Nisin and rosemary essential oil significantly increased the antioxidant and antibacterial abilities. The chitosan/Nisin/rosemary essential oil-blueberry anthocyanin (CSNR-ATH) film exhibited better UV barrier properties and lower water solubility. The CSNR-ATH film could reflect the freshness of clams sensitively during the refrigeration process, and the composite indicator film changed from light green to yellow-green. Therefore, the prepared smart indicator film (CSNR-ATH) provides a new choice for the freshness monitoring of clams.