目的 研究壳聚糖−罗伦隐球酵母生物活性膜处理对葡萄柚果实抗病保鲜及贮藏品质的影响。方法 以“里约红”葡萄柚为实验材料，采用壳聚糖(10 g/L)、罗伦隐球酵母发酵液(1×10⁷ CFU/mL)、壳聚糖−罗伦隐球酵母复合液对采后葡萄柚果实进行浸泡3 min处理，并设置CK组，在室温条件(温度为20 ℃±2 ℃，相对湿度为85%~90%)下贮藏。测定贮藏期间葡萄柚的果实发病率、果皮几丁质酶活性、β−1, 3−葡聚糖酶活性、质量损失率、果实外观色泽、营养物质、贮藏品质等指标。通过主成分综合评分法分析最佳的抗病保鲜方式。结果 在室温贮藏过程中，与CK组相比，壳聚糖、罗伦隐球酵母发酵液和壳聚糖−罗伦隐球酵母复合液处理组均能有效降低葡萄柚果实青霉病的发病率和病斑直径，在一定程度上诱导提高果皮抗病防御酶活性;抑制葡萄柚果实失水，增强果实的鲜亮度;延缓营养物质的流失。其中，壳聚糖−罗伦隐球酵母复合液处理对保持采后葡萄柚果实的贮藏品质最好。主成分分析结果表明，葡萄柚贮藏品质的综合得分顺序为壳聚糖−罗伦隐球酵母复合膜>CTS膜>C. laurentii膜>CK。结论 采用壳聚糖−罗伦隐球酵母生物活性膜处理对采后葡萄柚果实青霉病的抗病保鲜效果最好，能够明显降低果实的发病率，保持更好的贮藏品质。

The work aims to investigate the effects of chitosan-Cryptococcus laurentii yeast bioactive film coating treatment on disease resistance, preservation and quality of grapefruit during storage. With ''Rio Red'' grapefruit as the experimental material, harvested grapefruit was soaked respectively with 10 g/L chitosan, fermentation broth of Cryptococcus laurentii yeast (1×107 CFU/mL) and chitosan-Cryptococcus laurentii yeast complex solution for 3 min. A deionized-water-treatment group was set as the CK group, and then they were stored at room-temperature conditions (temperature:(20±2)℃, relative humidity:85%-90%). Quality indicators of fruits during storage such as disease incidence of fruits, chitinase and β-1,3-glucanase activity of peels, weight loss of fruits, color and nutrient quality were determined during storage. The best way of preservation was analyzed through the comprehensive principal component scoring method. During the storage at room temperature, the disease incidence and diameter lesions were effectively reduced in grapefruit through the treatment of chitosan, Cryptococcus laurentii and chitosan-Cryptococcus laurentii compared with the CK group. The activity of fruit peel resistance defense enzymes was enhanced to a certain extent after different treatments. These treatments also exhibited excellent preservation effects, such as the inhibition of weight loss, the enhancement of peel brightness and the delaying of nutrients loss. Among these treatments, with chitosan-Cryptococcus laurentii complex solution, the best postharvest quality of fruit was maintained. The results of principal component analysis showed that the comprehensive score of grapefruit storage quality was in the following order:chitosan-Cryptococcus laurentii composite film > CTS film > Cryptococcus laurentii film > CK film. Chitosan-Cryptococcus laurentii bioactive film treatment has the best effect on the disease resistance and preservation of green mold of harvested grapefruit, which can significantly reduce the incidence of disease and maintain fruit storage quality.