目的 优化用醋酸菌生产菌膜的最适培养条件和方法，以期获得天然、安全的食品包装用膜的生产工艺。方法 以涩柿醋为原料，分离筛选出高产菌膜的优良醋酸菌种，通过单因素和Plackett−Burman 等试验设计优化醋酸菌菌膜最适培养方法和工艺条件。结果 从涩柿醋中分离纯化获得产膜醋酸菌，经鉴定为葡糖醋杆菌。用醋酸菌生产醋酸菌菌膜最优培养条件:蔗糖质量分数为5%、蛋白胨质量分数为0.7%、酵母提取物质量分数为1.2%、柠檬酸钠质量分数为0.15%、K2HPO4质量分数为0.16%、乙酸体积分数为1%、无水乙醇体积分数为2%，种龄为32 h，接种量为9%;培养5 d后，醋酸菌菌膜的平均产率为364.65 g/L，是优化前的(246.03 g/L)的1.48倍。湿膜平均含水率为99.38%，复水率为71.09%～83.39%，菌膜干膜复水率高且复水速度快，复水1 min其含水率便可达到70%以上。结论 醋酸菌用经过优化选择的培养液和方法，可生产出高质量的菌膜。

The work aims to optimize the optimum culture conditions and methods for production of bacterial pellicle with acetic acid bacteria to obtain a natural and safe production process of pellicle for food packaging. Astringent persimmon vinegar was used as raw material, and excellent acetic acid bacteria with high yield membrane were isolated and screened. The optimum culture method and technological conditions of acetic acid pellicle were optimized by single factor and Plackett−Burman experimental design. The pellicle producing acetic acid bacteria was isolated and purified from astringent persimmon vinegar and identified as gluconacetobacter xylinum. The optimum conditions for culture of pellicle with acetic acid bacteria were as follows:sucrose 5% (mass fraction), peptone 0.7% (mass fraction), yeast extract 1.2% (mass fraction), sodium citrate 0.15% (mass fraction), K2HPO4 0.16% (mass fraction), acetic acid 1% (v/v), absolute ethanol 2% (v/v), seed age 32 h, inoculation amount 9%, cultured for 5 days. The average yield of pellicle was 364.65 g/L, which was 1.48 times as high as that before optimization (246.03 g/L). The average moisture content of wet pellicle was 99.38%, and the rehydration rate was 71.09%-83.39%. The rehydration rate of dry membrane was high and the rehydration speed was fast. The water content can reach more than 70% after one minute of rehydration. With the optimized culture medium and method, high-quality pellicle can be produced with acetic acid bacteria.