目的 低温休眠是鱼类保活运输中的关键技术之一，以金鲳鱼为对象，探讨海水温度对其保活过程中应激和代谢的影响，确定保活运输的重要温度参数。方法 对鱼体进行梯度降温使之休眠，在不同温度下进行保活，得出最佳保活温度，对保活过程中的各项指标进行测定。结果 在金鲳鱼保活过程中，鱼体代谢会使水体中氨氮总含量和pH值上升;金鲳鱼受低温胁迫会产生应激反应，血清的皮质醇含量上升，血糖含量下降，尿素氮和总胆固醇含量上升，乳酸脱氢酶活性稍有升高，谷草转氨酶活性显著升高，肌肉中糖原含量下降，乳酸含量上升;将金鲳鱼在(25±2)℃下暂养6 h后，梯度降温至13~14 ℃时金鲳鱼逐渐进入休眠状态，然后再移入15~17 ℃海水中进行保活;在15 ℃和17 ℃保活36 h存活率均为100%，保活48 h存活率分别为83%和75%。结论 海水温度对金鲳鱼保活过程中应激和代谢具有重要影响，且为金鲳鱼的有水保活运输提供了理论指导和关键温度参数。

Low temperature dormancy is one of the key technologies in fish keep-alive transportation. The influences of seawater temperature on the stress and metabolism of golden pompano (Trachinotus ovatus) were studied in the process of keep-alive transportation, to obtain the important temperature parameters for keep-alive transportation. Gradient cooling of the fish body was implemented to make it dormant, keep the fish alive at different temperatures, obtain the best keep-alive temperature, and measure various indicators in the keep-alive process. The results showed that the fish metabolism increased the total ammonia nitrogen content and pH value in seawater in the process of keep-alive transportation. Golden pompano had a stress response under low temperature:the cortisol content in serum increased, the blood sugar content decreased, the urea nitrogen and total cholesterol content increased, lactate dehydrogenase activity increased slightly, and aspartate aminotransferase activity increased significantly; the glycogen content in muscle decreased, and lactic acid content increased. Golden pompano was kept temporarily at (25±2)℃ for 6 h, and the temperature was gradually lowered to 13-14 ℃ , at this time, the fish was in a dormant state. Then the fish was moved to seawater of 15-17 ℃ to keep alive. The survival rates at 15 ℃ and 17 ℃ for 36 h were both 100%, and the survival rates for 48 h were 83% and 75% respectively. The results showed that seawater temperature has an important influence on the stress and metabolism of golden pompano. We can get theoretical guidance and key temperature parameters for the keep-alive transportation of golden pompano.