目的 以废弃鱿鱼顶骨为原料，在温和条件下提取并制备高强度、高透明度的β-甲壳素纳米纤维薄膜。方法 常温下利用温和提取法，从鱿鱼顶骨中提取β-甲壳素纳米纤维(β-ChNF);通过真空抽滤，制备β-甲壳素纳米纤维薄膜(β-ChNF/m)。并与以蟹壳为原料“高温提取”制备所得的α-甲壳素纳米纤维薄膜(α-ChNF/m)进行对比;通过力学性能、结晶结构、微观构造、表面化学特性及透光性分析，探索“温和条件”提取法对薄膜性能的影响规律。结果 利用温和提取法，能最大程度保留β-ChNF在鱿鱼顶骨中的天然纳米形态与性能;与从蟹壳中提取所得α-甲壳素相比，研究制备所得β-ChNF/m比α-ChNF/m力学强度提高了近1.5倍，断裂伸长率提高了3倍，且具备优异的透光性，透光率为90%，雾度为4%。结论 以鱿鱼顶骨为原料制备所得β-ChNF/m具有优异的透光性、力学性能，有望作为柔性电子标签基材应用于智慧包装、包装智能检测等研究领域。

This work aims to fabricate β-chitin nanofiber membranes with high transparency and mechanical properties from squid pen. By using a simple and mild condition, the β-chitin nanofibers were extracted from squid pen with high aspect ratio and good integrity. Then β-chitin nanofiber membrane and α-chitin nanofiber membrane were prepared through high temperature extraction and vacuum filtration, respectively. The effects of mild conditions on the properties of the two kind of chitin nanofiber membranes were investigated by comparing their mechanical properties, crystalline structure, microstructure, surface chemical properties and light transmittance. The results show that the light transmittance and haze of β-chitin nanofiber membrane can reach 90% and 4%, respectively. Mechanical tests show that tensile strength of β-chitin nanofiber membrane prepared from squid pen is nearly 1.5 times higher than that of α-chitin nanofiber membrane prepared from crab shell. Therefore, this biocompatible and biodegradable material with high light transmittance and mechanical properties is expected to be used as a flexible electronic label for intelligent detection of packaging and other fields.