超声处理控制鲜切甘薯褐变的生理机制

潘艳芳, 陈晓彤, 杨维巧, 贾晓昱, 张继明, 李喜宏

包装工程(技术栏目) ›› 2019 ›› Issue (9) : 1-5.

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包装工程(技术栏目) ›› 2019 ›› Issue (9) : 1-5. DOI: 10.19554/j.cnki.1001-3563.2019.09.001

超声处理控制鲜切甘薯褐变的生理机制

  • 潘艳芳1, 陈晓彤1, 贾晓昱1, 李喜宏1, 杨维巧2, 张继明3
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Physiological Mechanism for Browning Inhibition in Fresh-cut Sweet Potato by Ultrasound

  • PAN Yan-fang1, CHEN Xiao-tong1, JIA Xiao-yu1, LI Xi-hong1, YANG Wei-qiao2, ZHANG Ji-min3
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摘要

目的 针对甘薯鲜切后易氧化褐变问题,研究超声处理对鲜切甘薯酶促褐变影响的机制,确定最佳的超声处理时间。方法 甘薯经鲜切后,先在25 ℃,40 kHz下超声处理不同时间(0,5,10,20 min),然后测定其在4 ℃冷藏期间对甘薯色泽、丙二醛(MDA)含量、褐变相关酶活性及酚类底物含量的影响。结果 适宜时间的超声处理能有效保持鲜切甘薯的亮度和色度,抑制MDA的积累,钝化多酚氧化酶(PPO)和过氧化物酶(POD)的活性,激发苯丙氨酸解氨酶(PAL)活性,提高了甘薯总酚含量和抗氧化能力,延缓了褐变的发生。结论 以超声处理10 min的防褐变效果最佳,处理20 min会对甘薯组织造成损伤,从而加重褐变。

Abstract

The work aims to study the physiological mechanism of ultrasonic treatment on enzymatic browning in view of the oxidative browning of fresh-cut sweet potato and find the optimal ultrasonic treatment time. Fresh-cut sweet potato slices were treated with ultrasound for 0, 5, 10 and 20 min at frequency of 40 kHz and temperature of 25 ℃. The color, malondialdehyde content, browning related enzyme activities and total phenol content of fresh-cut sweet potato at 4 ℃ storage were tested. Moderate ultrasonic time effectively maintained the brightness and color of fresh-cut sweet potato, inhibited the accumulation of malondialdehyde, passivated the activity of polyphenol oxidase and peroxidase, stimulated the activity of phenylalanine ammonia-lyase, improved the total phenol content and antioxidant capacity, and ultimately delayed browning of fresh-cut sweet potato. The best anti-browning effect is achieved by ultrasonic treatment for 10 min, and ultrasonic treatment for 20 min will cause damage to sweet potato tissue and aggravate browning.

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潘艳芳, 陈晓彤, 杨维巧, 贾晓昱, 张继明, 李喜宏. 超声处理控制鲜切甘薯褐变的生理机制[J]. 包装工程(技术栏目). 2019(9): 1-5 https://doi.org/10.19554/j.cnki.1001-3563.2019.09.001
PAN Yan-fang, CHEN Xiao-tong, YANG Wei-qiao, JIA Xiao-yu, ZHANG Ji-min, LI Xi-hong. Physiological Mechanism for Browning Inhibition in Fresh-cut Sweet Potato by Ultrasound[J]. Packaging Engineering. 2019(9): 1-5 https://doi.org/10.19554/j.cnki.1001-3563.2019.09.001

基金

“十三五”国家重点研发计划(2018YFD0401305-5);天津市科技计划(18ZXRHNC00070,17PTWYHZ 00030);天津市食品冷链物流工程技术中心建设项目(20171204)

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