目的 选用聚乙二醇（PEG）-硫酸铵（(NH4)2SO4）双水相体系萃取虎舌红皂苷，并研究皂苷的稳定性。方法 以萃取率为指标，考察水浴时间、水浴温度、PEG平均分子量、PEG质量分数、(NH4)2SO4质量分数、体系pH值对萃取效果的影响，采用响应曲面法对萃取条件进行优化，并对虎舌红皂苷进行稳定性研究。结果 得到了最佳萃取条件，水浴时间为40 min，水浴温度为70 ℃，PEG600质量分数为25.0%，(NH4)2SO4质量分数为21.0%，pH值为6.20，此时虎舌红皂苷的萃取率为4.73%。研究表明，虎舌红皂苷适于保存在20 ℃以下、pH为6的低温微酸性环境中。结论 此体系的建立能有效萃取虎舌红皂苷，为其后续研究提供参考依据。

The paper aims to extract saponins from Ardisia mamillata by polyethylene glycol (PEG)-ammonium sulfate (NH4)2SO4 two-phase system to study the stability of saponin. The effects of water bath time, temperature, PEG average molecular weight, PEG mass fraction, (NH4)2SO4 mass fraction and pH on the extraction efficiency were observed with the extraction rate of saponins in the system as index. The optimum extraction conditions were optimized by response surface methods, and the stability was studied. The results showed that the water bath time was 40 minutes and the temperature was 70 ℃, PEG600 mass fraction, (NH4)2SO4 mass fraction and pH was 25.0%, 21% and 6.20, respectively. Under these conditions, the extraction rate of saponins was 4.73%. Ardisia mamillata saponins was suitable for preservation at temperatures below 20 ℃ and pH 6. The establishment of this system can effectively extract saponins from Ardisia mamillata and provide reference for its follow-up study.